Friday, February 17, 2012

FYP in My Style: FTC


FTC = Ferric thiocyanate test. Okay based on my simple minded explanation, this test is to determine the lipid peroxide inhibition. Usually it is used in diary product.
Okay the preparation!!!

Prepares:
1. 75% Ethanol
2. 30% of Ammonium thiocyanate
3. 0.02 M Ferrous chloride in 3.5% of 36% hydrochloric acid
4. 2.5% linoleic acid
5. 0.02 M phosphate buffer (pH 7)
6. Vitamin E (positive control)

Sample preparation:
(A) 4.0 mg of sample is diluted with 4.0 ml 99.5% Ethanol/absolute Ethanol put in Universal bottle. Mixture of 4.1 ml of 2.5% linoleic acid in 99.5% Ethanol, 8.0 ml of 0.02 M phosphate buffer (pH 7), and 3.9 ml of water were added into the same Universal bottle as sample and was placed in an oven at 40 °C in the dark.

(B) 9.7 ml of 75% Ethanol added with 0.1 ml of 30% ammonium thiocyanate put in  10 ml Falcon tube.

Procedures:
1. Start up the UV-Vis spectrophotometry and do setup. As for setup, used wave program application with absorbance 500 nm.
2. Absolute Ethanol is used as a blank while the mixture with 4.0 ml absolute ethanol as negative control.
3. 0.1 ml of A was pipette into B. 0.1 ml of 0.02 M ferrous chloride in 3.5% of 36% hydrochloric acid then was added.
4. After 3 minutes after ferrous chloride was added, absorbance was measured with UV-Vis.
5. Repeat 3 & 4 in every 24 hours interval until day after the sample absorbance reached its peak value and dropped.

*please refers to journal and consult with your supervisor frequently.
*you guys still can ask me if you don't understand anything..insyaAllah I'll answer it if it is within my knowledge (^-^)

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