Friday, February 17, 2012

FYP in My Style: FTC

FTC = Ferric thiocyanate test. Okay based on my simple minded explanation, this test is to determine the lipid peroxide inhibition. Usually it is used in diary product.
Okay the preparation!!!

1. 75% Ethanol
2. 30% of Ammonium thiocyanate
3. 0.02 M Ferrous chloride in 3.5% of 36% hydrochloric acid
4. 2.5% linoleic acid
5. 0.02 M phosphate buffer (pH 7)
6. Vitamin E (positive control)

Sample preparation:
(A) 4.0 mg of sample is diluted with 4.0 ml 99.5% Ethanol/absolute Ethanol put in Universal bottle. Mixture of 4.1 ml of 2.5% linoleic acid in 99.5% Ethanol, 8.0 ml of 0.02 M phosphate buffer (pH 7), and 3.9 ml of water were added into the same Universal bottle as sample and was placed in an oven at 40 °C in the dark.

(B) 9.7 ml of 75% Ethanol added with 0.1 ml of 30% ammonium thiocyanate put in  10 ml Falcon tube.

1. Start up the UV-Vis spectrophotometry and do setup. As for setup, used wave program application with absorbance 500 nm.
2. Absolute Ethanol is used as a blank while the mixture with 4.0 ml absolute ethanol as negative control.
3. 0.1 ml of A was pipette into B. 0.1 ml of 0.02 M ferrous chloride in 3.5% of 36% hydrochloric acid then was added.
4. After 3 minutes after ferrous chloride was added, absorbance was measured with UV-Vis.
5. Repeat 3 & 4 in every 24 hours interval until day after the sample absorbance reached its peak value and dropped.

*please refers to journal and consult with your supervisor frequently.
*you guys still can ask me if you don't understand anything..insyaAllah I'll answer it if it is within my knowledge (^-^)

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